SrasV1, Main, Exploration, bibRecord, 004D37

The papain-like protease of severe acute respiratory syndrome coronavirus has deubiquitinating activity

Identifieur interne : 004D37 ( Main/Exploration ); précédent : 004D36; suivant : 004D38

The papain-like protease of severe acute respiratory syndrome coronavirus has deubiquitinating activity

Auteurs : Naina Barretto [États-Unis] ; Dalia Jukneliene [États-Unis] ; Kiira Ratia [États-Unis] ; ZHONGBIN CHEN [États-Unis, République populaire de Chine] ; Andrew D. Mesecar [États-Unis] ; Susan C. Baker [États-Unis]

Source :

Journal of virology [ 0022-538X ] ; 2005.

RBID : Pascal:06-0040396

Descripteurs français

KwdFr :
- Cellules HeLa, Domaine catalytique, Données de séquences moléculaires, Humains, Papaïne (), Papaïne (métabolisme), Peptide hydrolases (génétique), Peptide hydrolases (métabolisme), Spécificité du substrat, Séquence d'acides aminés, Ubiquitine (métabolisme), Virus du SRAS (enzymologie), Virus du SRAS (génétique), Virus du SRAS (physiologie).
MESH :
- enzymologie : Virus du SRAS.
- génétique : Peptide hydrolases, Virus du SRAS.
- métabolisme : Papaïne, Peptide hydrolases, Ubiquitine.
- physiologie : Virus du SRAS.
Pascal (Inist)
- Cellules HeLa, Coronavirus, Domaine catalytique, Données de séquences moléculaires, Humains, Papain, Microbiologie, Papaïne, Spécificité du substrat, Séquence d'acides aminés, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Amino Acid Sequence, Catalytic Domain, Coronavirus, HeLa Cells, Humans, Microbiology, Molecular Sequence Data, Papain, Papain (chemistry), Papain (metabolism), Peptide Hydrolases (genetics), Peptide Hydrolases (metabolism), SARS Virus (enzymology), SARS Virus (genetics), SARS Virus (physiology), Severe acute respiratory syndrome, Substrate Specificity, Ubiquitin (metabolism), Virology.
MESH :
- chemical , chemistry : Papain.
- chemical , genetics : Peptide Hydrolases.
- chemical , metabolism : Papain, Peptide Hydrolases, Ubiquitin.
- enzymology : SARS Virus.
- genetics : SARS Virus.
- physiology : SARS Virus.
- Amino Acid Sequence, Catalytic Domain, HeLa Cells, Humans, Molecular Sequence Data, Substrate Specificity.

Abstract

Replication of the genomic RNA of severe acute respiratory syndrome coronavirus (SARS-CoV) is mediated by replicase polyproteins that are processed by two viral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro). Previously, we showed that SARS-CoV PLpro processes the replicase polyprotein at three conserved cleavage sites. Here, we report the identification and characterization of a 316-amino-acid catalytic core domain of PLpro that can efficiently cleave replicase substrates in trans-cleavage assays and peptide substrates in fluorescent resonance energy transfer-based protease assays. We performed bioinformatics analysis on 16 papain-like protease domains from nine different coronaviruses and identified a putative catalytic triad (Cys1651-His1812-Asp1826) and zinc-binding site. Mutagenesis studies revealed that Asp1826 and the four cysteine residues involved in zinc binding are essential for SARS-CoV PLpro activity. Molecular modeling of SARS-CoV PLpro suggested that this catalytic core may also have deubiquitinating activity. We tested this hypothesis by measuring the deubiquitinating activity of PLpro by two independent assays. SARS CoV-PLpro hydrolyzed both diubiquitin and ubiquitin-7-amino-4-methylcoumarin (AMC) substrates, and hydrolysis of ubiquitin-AMC is approximately 180-fold more efficient than hydrolysis of a peptide substrate that mimics the PLpro replicase recognition sequence. To investigate the critical determinants recognized by PLpro, we performed site-directed mutagenesis on the P6 to P2' residues at each of the three PLpro cleavage sites. We found that PLpro recognizes the consensus cleavage sequence LXGG, which is also the consensus sequence recognized by cellular deubiquitinating enzymes. This similarity in the substrate recognition sites should be considered during the development of SARS-CoV PLpro inhibitors.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316023

Affiliations:

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
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<term>SARS Virus (genetics)</term>
<term>SARS Virus (physiology)</term>
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<term>Ubiquitin (metabolism)</term>
<term>Virology</term>
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<term>Virus du SRAS (génétique)</term>
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<front><div type="abstract" xml:lang="en">Replication of the genomic RNA of severe acute respiratory syndrome coronavirus (SARS-CoV) is mediated by replicase polyproteins that are processed by two viral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro). Previously, we showed that SARS-CoV PLpro processes the replicase polyprotein at three conserved cleavage sites. Here, we report the identification and characterization of a 316-amino-acid catalytic core domain of PLpro that can efficiently cleave replicase substrates in trans-cleavage assays and peptide substrates in fluorescent resonance energy transfer-based protease assays. We performed bioinformatics analysis on 16 papain-like protease domains from nine different coronaviruses and identified a putative catalytic triad (Cys1651-His1812-Asp1826) and zinc-binding site. Mutagenesis studies revealed that Asp1826 and the four cysteine residues involved in zinc binding are essential for SARS-CoV PLpro activity. Molecular modeling of SARS-CoV PLpro suggested that this catalytic core may also have deubiquitinating activity. We tested this hypothesis by measuring the deubiquitinating activity of PLpro by two independent assays. SARS CoV-PLpro hydrolyzed both diubiquitin and ubiquitin-7-amino-4-methylcoumarin (AMC) substrates, and hydrolysis of ubiquitin-AMC is approximately 180-fold more efficient than hydrolysis of a peptide substrate that mimics the PLpro replicase recognition sequence. To investigate the critical determinants recognized by PLpro, we performed site-directed mutagenesis on the P6 to P2' residues at each of the three PLpro cleavage sites. We found that PLpro recognizes the consensus cleavage sequence LXGG, which is also the consensus sequence recognized by cellular deubiquitinating enzymes. This similarity in the substrate recognition sites should be considered during the development of SARS-CoV PLpro inhibitors.</div>
</front>
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<name sortKey="Zhongbin Chen" sort="Zhongbin Chen" uniqKey="Zhongbin Chen" last="Zhongbin Chen">ZHONGBIN CHEN</name>
</country>
<country name="République populaire de Chine"><noRegion><name sortKey="Zhongbin Chen" sort="Zhongbin Chen" uniqKey="Zhongbin Chen" last="Zhongbin Chen">ZHONGBIN CHEN</name>
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The papain-like protease of severe acute respiratory syndrome coronavirus has deubiquitinating activity

The papain-like protease of severe acute respiratory syndrome coronavirus has deubiquitinating activity

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